Following the outbreak of the Second World War, 92 Squadron reformed on 10 October 1939 at RAF Tangmere. Initially it flew Bristol Blenhiems but in early 1940 it became operational on the Supermarine Spitfire, various marks of which it flew for the rest of the War. 92 Squadron first saw action over the Dunkirk evacuation beaches. During the latter stages of the Battle of Britain 92 Squadron flew from RAF Biggin Hill. The Squadron was the first into action on September 15, 1940 now known as Battle of Britain Day.
In February 1942, the Squadron was posted to Egypt to join Air Headquarters Western Desert to support the Allies on the ground. Personnel arrived in Egypt in April but no aircraft were available. Some pilots flew operations with Hurricane of No. 80 Squadron. Spitfires finally arrived in August and the squadron commenced operations from RAF Heliopolis over the El Alamein sector, and then with their Spitfire Vs at Landing GRound 173 in the Western Desert. No. 92 Squadron provided air cover at the Battle of El Alamein and on April 18, 1943, 11 Spitfires from the squadron flew top cover at the Palm Sunday Massacre during which approximately 75 axis aircraft were disabled or destroyed.Following the Allied victory in North Africa, the Squadron moved to Malta in June. It went on to provide air cover for the 8th Army during the campaigns in Sicily and Italy, arriving on Italian soil on 14 September 1943. No. 92 Squadron then followed the armies up the Italian coast as part of No. 244 Wing and No. 211 (Offesnive Fighter) Group. During World War II the Squadron claimed the highest number of victories scored, 317, in the RAF.